Catalase is an example of an enzyme that speeds up the process of reducing hydrogen peroxide (Urry, ). Cow catalase with iron cofactor and bacteria with iron cofactor are examples of enzymes that speed up the reduction of hydrogen peroxide. In our experiment however, we narrowed our study to the cow catalase with the iron cofactor If you need professional help with completing any kind of homework, blogger.com Catalase Hydrogen Peroxide Experiment Coursework is the right Catalase Hydrogen Peroxide Experiment Coursework place to get the high quality for affordable prices. Whether you are looking for essay, coursework, research, or Catalase Hydrogen Peroxide Experiment Coursework Coursework Catalase homework help service should ever provide a student with just any college assignment assistance. Coursework Catalase This choice should be up to you! With us you are in control. You tell us how you want your college assignment to be done and we listen to all instructions and work on the paper according Coursework Catalase to them. Our high-quality, but cheap assignment /10()
in a catalase controlled experiment, the negative control can be shown by? - King Essays
I am experienced in the fields of biology, physics and chemistry, and I enjoy writing on these topics, coursework catalase. Note: This coursework catalase an A-level piece of coursework that achieved full marks. Catalase is an enzyme which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide into water and oxygen. Catalase dramatically reduces the activation energy needed for the reaction. Without catalase, coursework catalase, the decomposition would take much longer and would not be fast enough to sustain human life.
Hydrogen peroxide is also a dangerous, very potent by-product of metabolism, and it is essential that it is broken down quickly so it does not cause damage to cells, coursework catalase. Investigate the effect of substrate concentration on the rate of activity coursework catalase the enzyme catalase. I believe that as the concentration of the hydrogen peroxide substrate decreases, the rate of reaction will decrease as well.
This is because as there are progressively fewer molecules of hydrogen peroxide there will be fewer collisions between the substrate and enzyme molecules catalase coursework catalase yeastleading to a decrease in enzyme-substrate complexes formed.
Because the enzyme is the limiting factor, the reaction will stop completely when all of the active coursework catalase become saturated with substrate. This will result in a decreased volume of oxygen being produced as one of the by-products of this reaction.
In addition, based coursework catalase my knowledge of the collision theory I believe that if the concentration of hydrogen peroxide is doubled or halved then the rate of reaction is also doubled or halved.
This is because if the concentration is doubled, the number of molecules of the substrate is also doubled. This means there will be twice as many successful collisions, coursework catalase. Therefore it is true to say that in theory, rate µ concentration. As a result of my preliminary work, I have identified problems that may occur in my main investigation, such as timing, measuring and keeping variables that I am not investigating constant.
Here are the proposed solutions to the problems I identified. In the main procedure, I will control the temperature with a water coursework catalase in order to create a constant external temperature and dissipate the heat energy, coursework catalase.
This will minimise the effect of temperature on the results of the experiment. I have decided to do this because during my preliminary procedures I used a thermometer to measure the temperature of the hydrogen peroxide when left coursework catalase the side at different intervals and on different days, and I found that the temperature of the hydrogen peroxide fluctuated slightly.
By doing this, it will ensure that the test is as fair as I can make it. Although the reaction is exothermic and will give out heat during the reaction anyway, dissipating the heat with the water bath means the amount of heat given off in the experiment will be relative to the concentration of hydrogen peroxide.
Obviously, some reactions will take longer than others, so more heat will be produced, however, the initial temperature will be kept the same in each case.
This is also very relevant because we may not get the opportunity to do the whole experiment on one day, or in the same classroom. This means the room temperature in each classroom or on different days will not be the same for each procedure, because of obvious factors such as the type of day very cold or mild, etc, coursework catalase.
and the level of heating within the classrooms. Temperature directly affects the shape of the active site. At a temperature below the optimum, the molecules have less kinetic energy, so the rate of collisions between enzyme and substrate molecules is low, therefore less enzyme-substrate complexes are formed.
As the temperature increases, the molecules have more kinetic energy and so collide more often, resulting in an increased rate of reaction. Because of this, it is very important to ensure a constant temperature is maintained. Above the optimum temperature, the thermal energy breaks the hydrogen bonds holding the secondary and tertiary structure together, so the active site changes shape and eventually the reaction can no longer be catalysed.
I will keep the water bath at 25°C because the optimum temperature for the enzyme catalase is 45°C. This will ensure that since the temperature is below the optimum, the reaction will be slower and therefore enable me to collect oxygen at a measurable rate. I may, coursework catalase, however, need to change this as I have not done a preliminary experiment using a water bath, coursework catalase.
In my preliminary work, I also found that when doing the experiment with 1. I consequently reduced the mass of yeast to 0. This meant that because the enzyme concentration catalase in yeast was reduced, there were fewer collisions between enzyme and substrate molecules, coursework catalase, so the rate of enzyme-substrate formations was reduced.
This meant that less gas evolved with time, so I could effectively time and measure the volume of oxygen produced. Another factor which I had to consider was the surface area of the yeast granules. Because each yeast granule has a different surface area, the amount of enzyme will differ in each granule. More importantly, coursework catalase, the greater the surface area of the yeast, coursework catalase, the more reactions take coursework catalase because there will be more collisions between the enzyme and substrate molecules.
In my first preliminary experiment, I weighed 1. However, in my next preliminary experiment, I decided this would be unfair in the main procedure. Because of this, I decided to grind the yeast into a powder so that the surface area would be more similar in each yeast granule. Also, in my main procedure, coursework catalase, I will grind a larger mass of yeast more than I needand then weigh it, rather than coursework catalase the yeast and then grinding it.
This is important coursework catalase if I weigh the yeast and then grind it with the pestle, some of the yeast will be lost because it might get stuck to the pestle, hence slightly decreasing the mass of yeast. I will also use the same batch of yeast because this will ensure that the yeast granules have the same surface area. I also used two different methods in order to determine which would be coursework catalase most effective in gaining the best possible results with minimal error.
A syringe with hydrogen coursework catalase is also present as shown Fig. The hydrogen peroxide is injected into the test tube, and the volume of oxygen gas is recorded by the amount of water displaceddetermining the rate of reaction, coursework catalase. However, I decided against this method for several reasons.
Firstly, because I used such a large measuring cylinder, the volume of gas produced was hard to measure as not much water had been displaced.
Although I could have used a smaller measuring cylinder, I decided that the best possible way I could do the experiment was by measuring the volume of gas directly using a gas syringe, rather than by the displacement of water, coursework catalase. Also, because the hydrogen peroxide had to be inserted into the syringe before the reaction could begin, the amount of time it would be out of the water bath which I intend to use in my main experiment was longer than necessary.
I decided that I could reduce this time by using a different method. The hydrogen peroxide is inserted into a 5cm 3 beaker and then tipped coursework catalase to 'spill' the contents and start the reaction. I felt that this would give me more reliable results in my main investigation because the length of time that the hydrogen peroxide is out of the water bath is reduced.
Furthermore, the volume of gas is measured directly. I noticed that when doing the first method that 'bubbles of gas' were affected by people bumping the table, and that sometimes they got trapped in the tube, so even though the product of the coursework catalase oxygen had been formed, it was not measured until afterwards at a later stage in the reaction.
Also, the bubble volume is affected by the diameter of the tube and the overall pressure of the water depth so I believe that by using the gas syringe, I will be able to eliminate this inaccuracy as water will not be involved. The gas syringe, however, coursework catalase, has a small volume of air displaced within it when it is attached to the conical flask, so I will have to consider this in the main procedure. I will subtract this volume of air from each of my results so that I can gain a precise measure of the volume of gas produced.
My preliminary experiments also gave me an idea as to how often I should measure the volume of gas formed i. every 5, 10, 15 seconds etc.
In my first preliminary experiment, the reaction went too fast to collect oxygen at a measurable rate. In the second preliminary experiment, coursework catalase, I measured the volume of gas every 10 seconds but found that the reaction was over before I had enough measurements coursework catalase that the results I gained would not be sufficient to obtain enough data to make a valid conclusion.
Therefore I did a further experiment based on timing only and found that if I measured the volume of gas every 5 seconds I obtained enough measurements. However, I do have to take into account that I will be using different concentrations of hydrogen peroxide in my main experiment, so 5 seconds may not coursework catalase sufficient to measure the volume of oxygen produced in the slower reactions, coursework catalase, and I may need to change this.
The independent variable the factor that I manipulate will be the concentration of the hydrogen peroxide. I will put the 6 different concentrated solutions in a conical flask which will be placed in a water bath. Because a pipette is a very accurate way of measuring volumes, I believe that this will be the best method to make the concentrations. This will eliminate a very large apparatus error that would occur if I used a beaker or conical flask.
The dependent variable the one I intend to measure is the volume of gas produced in each reaction. This will vary as a direct result of the different concentrations of hydrogen peroxide. One such variable will be the mass of yeast for each experiment 0. I will make sure that I measure 0. The balance has a mechanism whereby it can be made level perfectly balanced regardless of the angle of the desk or counter it is placed on.
I have explained this in my coursework catalase below. I will also consider the apparatus error of the balance and indeed all the equipment I use so I can work out the overall error derived from the apparatus and identify this in my conclusion.
I am also controlling the temperature, coursework catalase. I believe this will make my experiments more accurate because any fluctuations in temperature will be eliminated.
It will also rule out the fact that if I have to do my procedures in different rooms and on different days, the temperature in the room might change. Coursework catalase 2.
Composition of hydrogen peroxide concentrations. Coursework catalase peroxide, if inhaled or in contact with the skin or eyes, can be coursework catalase dangerous and toxic.
For this reason, I will take the following safety precautions:. This is because there will coursework catalase more collisions between the enzyme and substrate molecules resulting in more enzyme-substrate complexes. The curve will then level off, representing the point where most of the enzymes' active sites are saturated.
The curve coursework catalase eventually plateau when the enzyme molecules have coursework catalase fully saturated. This is called the maximum velocity of the reaction or Coursework catalase. The substrate concentration at this point, even if increased, will not affect the rate of reaction because it is the enzyme which is in low concentration, coursework catalase.
This is because there will be fewer substrate molecules in each successive concentration, so fewer collisions between particles that can react with each other, coursework catalase. This means that the number of collisions that reach the activation energy also decreases, coursework catalase. I will record my results in a table like the one below, and then record further, average results, in a similar table, coursework catalase.
I will draw a graph based on the average results, coursework catalase, and draw a curve of best fit coursework catalase each concentration which will help coursework catalase analyse my results. I will then work out the gradient of each curve and plot a further graph of percentage of H 2 O 2 against the rate of reaction on the y-axis, coursework catalase.
I would expect this graph to be linear as this would show that as the concentration increases, coursework catalase, the time taken for a set volume of gas would decrease, coursework catalase. In other words, the rate is proportional to the concentration.
Test de la catalase
, time: 2:05Effect of Substrate Concentration on the Rate of Activity of Catalase - Owlcation
At, we Coursework On Catalase focus on building long-term, highly satisfactory relationships with all of our clients. You will never want to use another homework help service once you used ours. Trust some or all of your schoolwork to us and set yourself free from academic Coursework On Catalase stress. All you need to do is go online, give us a call or send a chat message and say: “Do my assignment”/10() If you want to offer some help with the writing process to be sure the work goes as expected, get in touch Catalase Hydrogen Peroxide Coursework with the writer whenever it is necessary. If you want to see our essay sample, Click Here. 15% off. Type of paper needed. Essay (any type) slide 4 to 5 of 4 No More Stress! We have Coursework On Catalase the solutions to your Academic problems. At we have a team of MA and PhD qualified Coursework On Catalase experts working tirelessly to provide high quality customized writing solutions to all your assignments including essays, term papers, research papers, dissertations, coursework and projects/10()
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